Cell populations are rarely homogeneous and synchronized in their characteristics. Single-cell RNA sequencing is a high-throughput tool for transcriptional profiling of thousands of individual cells. Recent advances in microdroplet-based approaches have made transcriptome analysis of tens of thousands of individual cells not only feasible, but not cost-prohibitive and the dataset are manageable to interpret. UF IMC Single-Cell RNA-Seq utilizes the latest technology on the market: the 10X Genomics Chromium™. This system provides a streamlined and reproducible workflow not typical of other single-cell RNA-seq assays.
SEQ_001: Encapsulation Training
Guided approach on encapsulating samples for single cell analysis using the 10X Genomics Chromium.