Service Request Form Start the Process How can we help? The UF IMC provides standardized, state-of-the-art immune monitoring assays at the RNA, protein, and cellular level, as well as archiving, reporting, and data mining support for clinical and translational studies. In partnership with the research community, we also work to test and develop new technologies for immune monitoring. Name Mr.Mrs.MissMs.Mx.Dr.Prof.Rev. Prefix First Last Email Institution or Company Name*Type of service(s) requested* BioBanking Flow Cytometry Data Collection Flow Cytometry Data Analysis Fluorescence-activated Cell Sorting (FACS) Genotyping Hands-on Training & Seminars Immunoassays (Luminex) Single-cell RNA-seq (using 10x Genomics Chromium) Project NameCourseAdvanced Flow Cytometry: Panel Design and AnalysisBio-Rad S3e Cell Sorter Operator TrainingFlowJo AnalysisFACSAria IIu/III Cell Sorter Operator TrainingIntro to Flow CytometryNumber of SamplesSample IDList a 3 digit sample ID for each sample.Number of Cells per SamplePlease enter a number from 100 to 20000.Depending on the heterogeneity of the sample and the depth of coverage you desire, this number can be as low as 100 or as high as 20,000. Number of Reads per cellFor the most applications an average of 50,000 reads per cell should be sequenced (for cell types with complex transcriptomes). For isolated nuclei 25,000 reads each are recommended,Population sorting or single-cell sorting?Population SortingSingle-cell SortingNumber of cellsThe total number of cells to be sorted that day (MUST be a number). Have you discussed your panel with the Core Manager? Yes, verbally or by email I sent it via Fluorofinder.com No Desired Date of Service Please give a date that you would like your requested service completed on. Deadline for DataIf there is a hard deadline for when you need the data for this project, please let us know. If the date is in less than 25 days, it may require an additional "rush" fee to move you forward in the queue. Method of Payment*UF Project Chart-FieldCheckUF Chart-Field or Project NumberPlease enter the UF Project Number that the funding will come from. If you don't know the project number, enter the phase you would provide to your admin for direction (e.g., "R01," "Flex," or "Pfizer")Checks are accepted!
